超高效液相色谱法研究新人参二醇在大鼠尿液中的排泄

耿聪，尹建元，刘继华，管清香，魏忠林，孙德亚，孟勤*

中国药学杂志 ›› 2013, Vol. 48 ›› Issue (8) : 636-639.

PDF(810 KB)
中国药学杂志
PDF(810 KB)
中国药学杂志 ›› 2013, Vol. 48 ›› Issue (8) : 636-639. DOI: 10.11669/cpj.2013.08.015
论著

超高效液相色谱法研究新人参二醇在大鼠尿液中的排泄

  • 耿聪1，尹建元1，刘继华1，管清香1，魏忠林2，孙德亚1，孟勤1*
作者信息 +

Urine Excretion of Neopanaxadiol in Rats by UPLC-MS/MS

  • GENG Cong 1, YIN Jian-yuan 1, LIU Ji-hua 1, GUAN Qing-xiang 1, WEI Zhong-lin 2,SUN De-ya 1,MENG Qin1*
Author information +
文章历史 +

摘要

目的 建立测定大鼠尿液中新人参二醇含量的UPLC-MS/MS分析方法，研究新人参二醇在大鼠尿液中的排泄情况。方法 尿液样品采用液-液萃取法进行提取，通过UPLC-MS/MS分析测定单剂量灌胃给予新人参二醇后大鼠尿液中原药的含量，计算尿液中原药的累积排泄量和平均排泄率。结果 新人参二醇在80~1 280 ng·mL-1呈现良好的线性关系，质控样品的日内和日间精密度（RSD）均小于15%，方法回收率均高于80%；大鼠灌胃新人参二醇(100 mg·kg-1)后，96 h内原药在尿中的累积排泄量占给药剂量百分比为0.023 3%。结论 新人参二醇几乎不以原型的形式从尿中排泄。

Abstract

OBJECTIVE To develop a UPLC-MS/MS method for the determination of neopanaxadiol (NPD) in rat urine samples， and to explore the excretion patterns of NPD in rats after oral administration. METHODS NPD was extracted from urine samples by liquid-liquid extraction. The concentration of NPD in urine was determined by UPLC-MS/MS and the cumulative excretion amount and excretion rate of NPD were calculated after a single oral administration of NPD at 100 mg·kg-1 to SD rats. RESULTS Excellent linearity was found between 80-1 280 ng·mL-1. The intra- and inter-day RSDs of the QC samples were both below 15% and the extraction recoveries of NPD were higher than 80%. The cumulative excretion of unchanged NPD in urine within 96 h amounted to （0.023 3±0.035 6）% of the dose. CONCLUSION NPD is hardly eliminated through urine within 96 h in rats.

关键词

超高效液相色谱质谱法 / 新人参二醇 / 尿液排泄

Key words

UPLC-MS/MS / neopanaxadiol (NPD) / urine excretion

引用本文

EndNote

Ris (Procite)

Bibtex

导出引用
耿聪，尹建元，刘继华，管清香，魏忠林，孙德亚，孟勤*. 超高效液相色谱法研究新人参二醇在大鼠尿液中的排泄[J]. 中国药学杂志, 2013, 48(8): 636-639 https://doi.org/10.11669/cpj.2013.08.015
GENG Cong , YIN Jian-yuan , LIU Ji-hua , GUAN Qing-xiang , WEI Zhong-lin ,SUN De-ya ,MENG Qin*. Urine Excretion of Neopanaxadiol in Rats by UPLC-MS/MS[J]. Chinese Pharmaceutical Journal, 2013, 48(8): 636-639 https://doi.org/10.11669/cpj.2013.08.015
中图分类号： R917   

参考文献

TAO L N， MENG Q， YIN J Y， et al. A new panaxadiol from the acid hydrolysate of Panax ginseng . Chin Chem Lett， 2009， 20(6)687-689.
YIN J Y， MENG Q， TAO L N. A method for preparation of a new panaxadiol and derivatives and pharmaceutical applicationChina， ZL200710055693.4 . 2009-07-01.
JOO K M， LEE J H， JEON H Y， et al. Pharmacokinetic study of ginsenoside Re with pure ginsenoside Re and ginseng berry extracts in mouse using ultra performance liquid chromatography/mass spectrometric method . J Pharm Biomed Anal， 2010， 51(1)：278-283.
LIN L， LIU J X， ZHANG Y， et al. Pharmacokinetic studies of ginsenoside Rg1， Re， Rb1 and Rd in rats by LC-MS/MS method . Chin Pharm J（中国药学杂志）， 2009，44(5)：373-377.
WANG W Y， SHAO Y F， MA S S， et al. Determination of 20(S)-protopanaxadiol ocotillol type epimers in rat plasma by liquid chromatography tandem mass spectrometry . J Chromatogr B， 2012， 887-888：19-24.
CHAMBERS E， WAGROWSKI-DIEHL D M， LU Z L， et al. Systematic and comprehensive strategy for reducing matrix effects in LC-MS/MS analyses . J Chromatogr B， 2007， 852(1-2)：22-34.
FDA. Guidance for IndustryBioanalytical Method Validation . 2001.

基金

国家自然科学基金资助项目(31070308)；“十一五”国家科技支撑计划重大项目(2009ZX09103-365)
PDF(810 KB)

57

Accesses

0

Citation

Detail
段落导航
相关文章

/

〈 〉